Leucine Rich Repeat and Coiled-Coil Domain Containing 1 (LRRCC1) (AA 1-1026) protein (Strep Tag)

Catalog No. ABIN3152668

Product Details

Target: Leucine Rich Repeat and Coiled-Coil Domain Containing 1 (LRRCC1)

Protein Type: Recombinant

Protein Characteristics: AA 1-1026

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: Strep Tag

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MEAAVCSEIE REDGDSSCGD VCFMDKGLHS ISELSLDSSI HAINLHCNNI SKISSIDHIW NLRHLDLSSN QISQIEGLNT LTKLCTLNLS CNLITRVEGL EALVNLTKLN LSYNHINDLS GLMPLHGLKY KLRYIDLHSN YIDSIHHLLQ CTVGLHFLTN LILEKDGEGN PICLIPGYRA IILQTLPQLR ILDCKNIFGE PVSLEEINSS HLQCLEGLLD NLVSSDSPLN ISEDEVNDDV PAPPMDVLPS LKEFKSTPED NVLASLLSVC PSSEPEKINQ ENDFQNEVKL QKLDDQILQL LNETNNSLID NVPEKDLRPK RDTDITSESD YGNRRECSRK VPRRTKIPYY ARTIQTIKHH NKNNGAFVSC NRKMRQPYLR DLYVRSSLVN CNNLRDLDEQ KTGVIKVDKN FSDNSTYRSL VEQLDQEREM RWKAEQTEKK LMDYIDELHK QADEKKDVHS QALITTDRLK DAIFKERHCK AQLEIIVHRL QNEVKKLTIE LMKARDQQED HIRHLRTLER ALEKMEKQKA QQQAAQIRLI QEVELKASAA DREINLLRTS LHQEKQQVQQ LHELLALKEQ EHRQEIETRQ FFTDAEFQDA LTKRLCKEER KHEQEVKEYQ EKIDILNQQY LDLENEFRIA LTVEARRFKD VQDGFEDVAT ELAKSKHALI WAQRKENESS SLIKDLTCMV KEQKTKLSEV CKLKQEAAAN LQNQINTLEI LIEDDKQKSI QIELLKHEKT QLISELAAKE SLIYGLRTER KVWGQELACQ SSTLSQSRGK LEAQIESLCR ENESLRKSHE SDCDALRIKC KIIEDQNETI RKLKDSLQEK DGQIKLLQEQ IALIEKCSQE QLNEKSSQLD SIVEKLERHN ERKEKLKQQL KAKELELEEI RKAYSTLNKK WHDKGELLSH LEMQVKEVKE KFEDKERKLK AERDKSLELQ KDAMEKLQNM DDAFRRQVDE IVEAHQAEIM QLANEKQKYI DCANLKVQQV EDEMRGLLDE TCKNKKMMEE KIKQLACAIS EIQKEM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Leucine Rich Repeat and Coiled-Coil Domain Containing 1 (LRRCC1)

Alternative Name: Lrrcc1 (LRRCC1 Products)

Synonyms: Sap2 Protein, MGC83921 Protein, im:7144254 Protein, si:ch211-106h4.13 Protein, CLERC Protein, SAP2 Protein, VFL1 Protein, 1200008A14Rik Protein, 4932441F23Rik Protein, AI195358 Protein, AI447421 Protein, leucine rich repeat and coiled-coil centrosomal protein 1 Protein, leucine rich repeat and coiled-coil centrosomal protein 1 L homeolog Protein, leucine rich repeat and coiled-coil domain containing 1 Protein, Lrrcc1 Protein, LRRCC1 Protein, lrrcc1.L Protein, lrrcc1 Protein

Background: Leucine-rich repeat and coiled-coil domain-containing protein 1,FUNCTION: Required for the organization of the mitotic spindle. Maintains the structural integrity of centrosomes during mitosis (By similarity). {ECO:0000250}.

Molecular Weight: 119.2 kDa

UniProt: Q69ZB0