TLR13 Protein (AA 69-991) (rho-1D4 tag)

Catalog No. ABIN3135832

Product Details

Target: TLR13 (Tlr13) (Toll-Like Receptor 13 (Tlr13))

Protein Type: Recombinant

Protein Characteristics: AA 69-991

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This TLR13 protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys), ELISA

Sequence: YGFNKCTQYE FDIHHVLCIR KKITNLTEAI SDIPRYTTHL NLTHNEIQVL PPWSFTNLSA LVDLRLEWNS IWKIDEGAFR GLENLTLLNL VENKIQSVNN SFEGLSSLKT LLLSHNQITH IHKDAFTPLI KLKYLSLSRN NISDFSGILE AVQHLPCLER LDLTNNSIMY LDHSPRSLVS LTHLSFEGNK LRELNFSALS LPNLTNLSAS RNGNKVIQNV YLKTLPQLKS LNLSGTVIKL ENLSAKHLQN LRAMDLSNWE LRHGHLDMKT VCHLLGNLPK LETLVFQKNV TNAEGIKQLA KCTRLLFLDL GQNSDLIYLN DSEFNALPSL QKLNLNKCQL SFINNRTWSS LQNLTSLDLS HNKFKSFPDF AFSPLKHLEF LSLSRNPITE LNNLAFSGLF ALKELNLAAC WIVTIDRYSF TQFPNLEVLD LGDNNIRTLN HGTFRPLKKL QSLILSHNCL KILEPNSFSG LTNLRSLDLM YNSLSYFHEH LFSGLEKLLI LKLGFNKITY ETTRTLQYPP FIKLKSLKQL NLEGQRHGIQ VVPSNFFQGL GSLQELLLGK NPSVFLDHHQ FDPLINLTKL DISGTKDGDR SLYLNASLFQ NLKRLKILRL ENNNLESLVP DMFSSLQSLQ VFSLRFNNLK VINQSHLKNL KSLMFFDVYG NKLQCTCDNL WFKNWSMNTE EVHIPFLRSY PCQQPGSQSL LIDFDDAMCN FDLGKVYFLC SFSMVLSTMV FSWFSTKMIA SLWYGLYICR AWYLTKWHKT EKKFLYDAFV SFSATDEAWV YKELVPALEQ GSQTTFKLCL HQRDFEPGID IFENIQNAIN TSRKTLCVVS NHYLHSEWCR LEVQLASMKM FYEHKDVIIL IFLEEIPNYK LSSYHRLRKL INKQTFITWP DSVHQQPLFW ARIRNALGKE TVEKENTHLI VVE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Tlr13 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TLR13 (Tlr13) (Toll-Like Receptor 13 (Tlr13))

Alternative Name: Tlr13 (Tlr13 Products)

Synonyms: RGD1562655 Protein, AI666735 Protein, Gm713 Protein, toll-like receptor 13 Protein, toll-like receptor13 L homeolog Protein, Toll-like receptor 13 Protein, Tlr13 Protein, tlr13.L Protein, tlr13 Protein

Background: Component of innate and adaptive immunity that recognizes and binds 23S rRNA from bacteria. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Specifically binds the 5'-CGGAAAGACC-3' sequence on bacterial 23S rRNA, a sequence also bound by MLS group antibiotics (including erythromycin). May also recognize vesicular stomatitis virus, however, these data require additional evidences. {ECO:0000269|PubMed:21131352, ECO:0000269|PubMed:22821982, ECO:0000269|PubMed:22896636}.

Molecular Weight: 107.9 kDa Including tag.

UniProt: Q6R5N8

Pathways: Activation of Innate immune Response