CC2D2A Protein (AA 1-1633) (Strep Tag)

Catalog No. ABIN3153894

Product Details

Target: CC2D2A (Coiled-Coil and C2 Domain Containing 2A (CC2D2A))

Protein Type: Recombinant

Protein Characteristics: AA 1-1633

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CC2D2A protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MNTNDEKMKI ISEDFTGDGV DTEAGRRKKN SKVRRQQRKK KPPASPPEEM VSDKFQDGGQ QEVVEEEPET NLLSLTARRG PRSLPPIPSA SRTGFAEFSM RERMREKLQA ARSKAESALL RDVPTPRPRR LRSPSREKET ETEFGTEPST EVQDTQKEDD TKSYSRIKFR DSVRKIKSKP QLPPGFPSAE EAYNFFTFNF DPEPEESEEK SPVKGGERAH HEDQEGEEGT QAQERAKKTE EEELLNGKDA EDFLLGLDPT AHDFVAVRAA EYKSARIQLQ KEKEILFTPS RLTVPTYKKL PENIQPRFLE DEGLYIGARP EVARTNENIM ENRLLIQEPG SKWFGDDGRI LALPSPIKPF PSRPSLTTRE QSPKAGLETL YKKAEKYVHS RQHMIGSGDP PGNFQLDIDI SGLIFTHHPC FSREHVLASK LAQLYDQYLA RQQRNKTKFL TDKLQALRKA VQTSLNPEKP HQSLDTTQKT INEYKSEIRQ TRKLRDAEQE KDRTLLKTII KVWKEMKSLR EFQRFTNTPL KLVLRKEKVD PKLDEDAYEA EIQAEIHELL EEHMEEYATK MEEYRTSHQQ WKAWRKAQRA KKKKKKQTTE EHLEEEEAEE SFPEEEVTKP IPPEPTDPAV IEQQVRERAA HSRRRPGEPT LIPELSLAGN VTPNDQCPRV EVSRREDVRR RSVYLKVVFN SKEVSRTVSR PLGADFRVHF GQIFNLQIFN WPESLMLQVY ETIGHSGTTL LAEVFLPIPE TTLVTGRAPI EEVEFSSNQH VTLDHEGVGS GVPFSFEADG SNQLTLMTSG KVSHSVAWAV GENGIPLIPP LSQQNIGFRS ALRRADAISS IGTSGLTDMK KLAKWAAESK LDPNDPNHAP LMQLISVATS GESYVPDFFR LEQLQQEFNF VSEEELNRSK RFRLLHLRSQ EVPEFRNYKQ IPAYDREIME KVFQDYEKRL RDRNVIETKD HLDMHRATVA KYLQQVREAV VNRFLTAKHH FLLTDLVVEE EVPNISSEGS GILGLSLFKL AEQKRPLRPR RKGRKKVTAQ NLSDGDIKLL VNIIRAYDIP VRKPVVSKFQ QPSRSSRTFS EKQTASPSTH SPLHNADYPL GQVLVRPFVE VSFQRTICHT TTAEGPNPSW NEELELPFRA PNGDYSTASL QSVKDDVYIN IFDEVLYDIL EDDRERGSGI HTRIERHWLG CVKIPFSTIY FQARIDGTFK IDIPPVLLGY SKERNIIMER AFDSARSLSE GSYITLFITI EPQLVPGEPM REKMSDMLKK FDTQEDEKLL QATEKFQAEC ALKFPQRQCL TTVTDMTGKT VFITRYLKPL NPPQELLHVY PNNPQATAEL VARYVSLIPF LPDSVSFAGV CDLWSTSDQF LDLLAGDEEE HAVLLCNYFL FLGKKAWLVM GSAIPEGPTA YVLTWEKNYY LIWNPCSGHC YGQFDAFCPL KSVGCLIGPD NIWFNIQHHD SPLRINFDVT KPKLWKSFFS RSLPYPGLSS VQPEELIYQH TDKAVAAELQ DRIEKILKEK IMDWRPRHLT RWNRYCTSTL RHFLPLLERS QGEDIEDDHR AELLKQLGDY RFSGFPLHMP YSEVKPLVEA VYSTGVHNID LPNVEFALAV YIHPYPKNVL SVWIYVASLV RNR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CC2D2A (Coiled-Coil and C2 Domain Containing 2A (CC2D2A))

Alternative Name: Cc2d2a (CC2D2A Products)

Synonyms: JBTS9 Protein, MKS6 Protein, 5730509K17Rik Protein, b2b1035Clo Protein, fc03c12 Protein, wu:fc03c12 Protein, RGD1561042 Protein, coiled-coil and C2 domain containing 2A Protein, coiled-coil and C2 domain containing 2A L homeolog Protein, CC2D2A Protein, Cc2d2a Protein, cc2d2a.L Protein, cc2d2a Protein

Background: Coiled-coil and C2 domain-containing protein 2A,FUNCTION: Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling. {ECO:0000269|PubMed:21725307, ECO:0000269|PubMed:22179047}.

Molecular Weight: 187.5 kDa

UniProt: Q8CFW7