GPS1 Protein (AA 1-471) (Strep Tag)

Catalog No. ABIN3154659

Product Details

Target: GPS1 (G Protein Pathway Suppressor 1 (GPS1))

Protein Type: Recombinant

Protein Characteristics: AA 1-471

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This GPS1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MQIDVDPQED PQNAPDVNYV VENPTLDLEQ YAASYSGLMR IERLQFIADR CPPLRVEALK MALSFVQRTF NVDMYEEIHR KLSEATRELQ NAPDAIPESG VEPPPLDTAW VEATRKKALL KLEKLDTDLK NYKGNSIKES IRRGHDDLGD HYLDCGDLSN ALKCYSRARD YCTSAKHVIN MCLNVIKVSV YLQNWSHVLS YVSKAESTPE IAEQRGERDS QTQAILTKLK CAAGLAELAA RKYKQAAKCF LLASFDHCDF PELLSPSNVA VYGGLCALAT FDRQELQRNV ISSSSFKLFL ELEPQVRDII FKFYESKYAS CLKMLDEMKD NLLLDMYLAP HVRTLYTQIR NRALIQYFSP YVSADMHKMA AAFNTTVAAL EDELTQLILE GLINARIDSH SKILYARDVD QRSTTFEKSL LMGKEFQRRA KAMILRAAVL RNQIHVKSPP REGSQGELTP ANSQSRMSTN M
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GPS1 (G Protein Pathway Suppressor 1 (GPS1))

Alternative Name: Gps1 (GPS1 Products)

Synonyms: COPS1 Protein, CSN1 Protein, Cops1 Protein, Csn1 Protein, R75577 Protein, Sgn1 Protein, im:6909260 Protein, im:7145572 Protein, zgc:154082 Protein, csn1 Protein, ARABIDOPSIS THALIANA FUSCA 6 Protein, ATFUS6 Protein, ATSK31 Protein, CONSTITUTIVE PHOTOMORPHOGENIC 11 Protein, COP11 Protein, COP9 SIGNALOSOME SUBUNIT 1 Protein, EMB78 Protein, EMBRYO DEFECTIVE 78 Protein, FUSCA 6 Protein, SHAGGY-LIKE KINASE 31 Protein, SK31 Protein, G protein pathway suppressor 1 Protein, casein alpha s1 Protein, G protein pathway suppressor 1 L homeolog Protein, COP9 signalosome complex subunit 1 Protein, GPS1 Protein, CSN1S1 Protein, LOC693104 Protein, CC1G_07518 Protein, Bm1_55055 Protein, Gps1 Protein, gps1 Protein, gps1.L Protein, FUS6 Protein, csn-1 Protein

Background: COP9 signalosome complex subunit 1 (SGN1) (Signalosome subunit 1) (G protein pathway suppressor 1) (GPS-1) (JAB1-containing signalosome subunit 1),FUNCTION: Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein- and mitogen-activated protein kinase-mediated signal transduction (By similarity). {ECO:0000250}.

Molecular Weight: 53.4 kDa

UniProt: Q99LD4

Pathways: Cell Division Cycle