RRBP1 Protein (AA 1-1605) (Strep Tag)

Catalog No. ABIN3137003

Product Details

Target: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1605

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RRBP1 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequence: MDIYDTQTLG VVVFGGFMVV SAIGIFLVST FSMKETSYEE ALANQRKEMA KTHHQKGEKK KKEKTVEKKG KTKKKEEKPN GKIPEHDLDP NVTIILKEPV RVSAVAVAPT SVHSSVGHTP IATVPAMPQE KLASSPKDRK KKEKKVAKVE PAVSSIVNSI QVLASKSAIL EATPKEVPMV AVPPVGSKAS SPATSSQGKK GQGAQNQAKK GEGAQNQGKK GEGAQNQAKK GEGAQNQAKK GEGAQNQGKK GEGAQNQAKK GEGGQNQAKK GEGAQNQGKK GEGAQNQGKK GEGAQNQAKK GEGAQNQAKK GEGAQNQGKK GEGAQNQSKK GEGAQNQAKK GEGGQNQAKK GEGAQNQAKK GEGAQNQAKK GEGVQNQAKK GVEGAQNQGK KGEANQNQAK KGEGGQNQTK KGEGPQNQGK KGEAAQKQDK KIEGAQNQGK KPEGTSNQGK KGEGAQNQGK KGEGAQNQSK KGEGAQNQAK KGEGGQNQAK KGEGAQNQAK KGEGAQNQAK KGEGVQNQAK KGVEGAQNQG KKGEANQNQA KKGEGGQNQT KKGEGPQNQG KKGEAAQKQD KKIEGAQNQG KKPEGTSNQG KKGEGAQNQG KKGEGAQNQG KKGEGAQNQG KKGEGAQNQG KKGEGAQNQG KKGEGAQNQG KKGEGAQNQG KKGEGPQNQA KKGEGAQNQG KKGEGAQNQG KKGEGAQNQG KKAEGVQSQS KKGEGTQNQG KKGDGNPNQG KKGEGASNQN RKTDTVANQG TKQEGVSNQV KKSEGSPNQG KKAEGAPNQG KKKDGSPSQA KKVDAAANQG KKSEMAPAQG QKASMVQSQE APKQDAPAKK KSGSRKKGEP GPPDCDGPLF LPYKTLVSTV GSMVFSEGEA QRLIEILSEK TGVIQDTWHK ATQKGDPVAI LKRQLQEKEK LLATEQEDAA VAKSKLRELN KEMASEKAKA AAGEAKVKKQ LVAREQEIAA VQARMQASYR DHVKEVQQLQ GKIRTLQEQL ENGPNTQLAR LQQENSILRD ALNQATSQVE SKQNTELAKL RQELSKVNKE LVEKSEASRQ EEQQRKALEA KAATFEKQVL QLQASHKESE EALQKRLEEV TRELCRAQTS HANLRADAEK AQEQQQRVAE LHSKLQSSEV EVKSKCEELS SLHGQLKEAR AENSQLTERI RSIEALLEAG QAQDTQASHA EANQQQTRLK ELESQVSCLE KETSELKEAM EQQKGKNNDL REKNWKAMEA LALAERACEE KLRSLTQAKE ESEKQLHLAE AQTKETLLAL LPGLSISAHQ NYAEWLQEFK EKGSELLKKP PTLEPSMDIV LKLREAEETQ NSLQAECDQY RTILAETEGM LKDLQKSVEE EERVWKAKVG AAEEELHKSR VTVKHLEDIV EKLKGELESS DQVREHTSHL EAELEKHMAA ASAECQNYAK EVAGLRQLLL ESQSQLDEAK SEAQKQSDEL ALVRQQLSDM RSHVEDGDVA GSPAVPPAEQ DPMKLKTQLE RTEATLEAEQ TRRQKLTAEF EEAQRTACRI QEELEKLRAA GPLESSGKEE ITQLKERLEK EKRLTSDLGR AAIKLQELLK TTQEQLTKEK DTVKKLQEQL GKAEDGSSSK EGTSV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Alternative Name: Rrbp1 (RRBP1 Products)

Synonyms: 1700087N07Rik Protein, 5730465C04Rik Protein, ES/130 Protein, mKIAA1398 Protein, mRRp0 Protein, mRRp1.8 Protein, mRRp10 Protein, mRRp15a Protein, mRRp15b Protein, mRRp16.8 Protein, mRRp2 Protein, mRRp41 Protein, mRRp47 Protein, mRRp5.4 Protein, p180 Protein, ES130 Protein, RRp Protein, hES Protein, P180 Protein, rrbp1 Protein, sb:cb489 Protein, wu:fc47a01 Protein, ribosome binding protein 1 Protein, ribosome binding protein 1 homolog 180kDa (dog) Protein, ribosome binding protein 1b Protein, Rrbp1 Protein, RRBP1 Protein, rrbp1b Protein

Background: Ribosome-binding protein 1 (Ribosome receptor protein) (RRp) (mRRp),FUNCTION: Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. {ECO:0000250}.

Molecular Weight: 172.9 kDa

UniProt: Q99PL5