Slco1a1 Protein (AA 1-670) (Strep Tag)

Catalog No. ABIN3130484

Product Details

Target: Slco1a1 (Solute Carrier Organic Anion Transporter Family, Member 1a1 (Slco1a1))

Protein Type: Recombinant

Protein Characteristics: AA 1-670

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This Slco1a1 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MEETEKKVAT QEGRFFSKMK VFLMSLTCAY LAKSLSGVYM NSMLTQIERQ FGIPTSVVGF ITGSFEIGNL LLIVFVSYFG RKLHRPIIIG VGCVVMGLGC FLMASPHFLM GRYKYETTIS PTSNLSSNSF LCIENRTQTL KPTQDPTECV KEIKSLMWIY VLIGNTMRGI GETPIMPLGI SYIEDFAKSE NSPLYIGILE MGKIVGPIIG LLLGSFFARV YVDIGSVNTD DLTITPTDTR WVGAWWIGFL VCAGVNILTS IPFFFFPKTL PKKELQDNVD VTKYEKVEKH RERAKKENLG ITKDFLPFMK SLCCNPIYML FSLTSVLQIN GFASTFTFLP KYLEQQYGKS TSEAVFLIGV YSLPPVCLGY LISGFIMKKF KITVKKAAYI AFGLSLSEYF IFLCNYLLTC DNFPVAGLTT SYKGVQHPLY GEKNVLADCN TRCSCLTDTW DPVCGDNGLA YMSACLAGCE KSVGTGTNMV FQNCSCIGSS GNSSAVLGLC KKGPECDNKL QYFLIKSVFS SFIFSLAAIP GYMVLLRCVK SEEKSIGVGL HAFFIRLLAG IPAPVYFGAL IDRTCLHWGT LKCGQPGACR MYDINRFRHI YLGLPAAVRG SSFLPAVFIL ILMRKFHFPG DIHSPDTELA EMKLTEKESE CTDVCRSPKV ENDGELKTKL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Slco1a1 (Solute Carrier Organic Anion Transporter Family, Member 1a1 (Slco1a1))

Alternative Name: Slco1a1 (Slco1a1 Products)

Synonyms: A530084B21 Protein, OATP-1 Protein, Oatp1 Protein, Oatp1a1 Protein, Slc21a1 Protein, Slc21a3 Protein, solute carrier organic anion transporter family, member 1a1 Protein, Slco1a1 Protein

Background: Solute carrier organic anion transporter family member 1A1 (Sodium-independent organic anion-transporting polypeptide 1) (OATP-1) (Solute carrier family 21 member 1),FUNCTION: Mediates the Na(+)-independent transport of organic anions such as steroid sulfate conjugates (dehydroepiandrosterone sulfate (DHEAS), 17-beta-glucuronosyl estradiol, estrone-3-sulfate), conjugated (taurocholate) and unconjugated (cholate) bile acids, prostaglandin E2 (PGE2) and L-thyroxine T4 (PubMed:10600646, PubMed:11267661). Also capable of transporting sulfobromophthalein (BSP), ouabain and gadoxetate (PubMed:10600646). Hydrogencarbonate/HCO3(-) acts as the probable counteranion that exchanges for organic anions (By similarity). Shows a pH -sensitive substrate specificity which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment (By similarity). {ECO:0000250|UniProtKB:P46720, ECO:0000269|PubMed:10600646, ECO:0000269|PubMed:11267661}.

Molecular Weight: 74.4 kDa

UniProt: Q9QXZ6