Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (Active) Protein

Details for Product No. ABIN3170543
Protein Name
Origin
Human
11
Source
Escherichia coli (E. coli)
7
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Positive Control (PC)
Options
Characteristics 5000 units/mg. One unit of the recombinant caspase-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM HEPES, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% glycerol and 10 mM DTT.
Purity 95 % by SDS-PAGE
Protein Name
Background Caspase-5 (also known as ICErelIII, TY) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-5 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. To date the mechanism of caspase-5 activation and roles of caspase-5 in apoptosis are poorly understood. The recombinant active human caspase-5 was expressed in E. coli. The active caspase-5 is routinely tested for its ability to enzymatically cleave these two substrates: Ac-WEHD-pNA and Ac-WEHD-AFC.
Application Notes Active caspase-5 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 1 unit per µL in PBS containing 15% glycerol.
Storage -80 °C