Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Protein

Details for Product No. ABIN3170544
Protein Name
Origin
Human
14
3
2
Source
Escherichia coli (E. coli)
17
1
1
Protein Type
Recombinant
Options
Characteristics 400 units/mg. One unit is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Purity 90 % by SDS-PAGE
Protein Name
Background Active enzyme was expressed in E. coli. The expressed caspase-9 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. One unit of rhCaspase-9 is the enzyme activity that cleaves 1 nmol of substrate Ac-LEHD-pNA per hour at 37°C at standard substrate concentrations. In combination with caspase-9 activity assays, the active rhCaspase-9 is useful in screening caspase inhibitors for pharmaceutical applications. The enzyme is also useful in studying enzyme regulation, determining target substrates and as a positive control in caspase-9 assays.
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity
Restrictions For Research Use only
Format Lyophilized
Storage -80 °C