Histidine-Rich Glycoprotein (HRG) (AA 1-525) (Active) protein (His tag)

Details for Product No. ABIN3216389
Protein Name
Protein Characteristics
AA 1-525
4
2
1
1
1
Origin
Human
8
4
3
1
Source
Human Cells
5
5
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Characteristics The secreted recombinant human HPRG comprises 518 amino acids with a predicted molecular mass of 59 kDa. As a result of glycosylation, rhHPRG migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human HPRG (NP_000403.1) (Met 1-Lys 525) with a C-terminal polyhistidine tag was expressed.
Predicted N-Term: Val 19
Purity > 97 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. (Lamb-Wharton, R.J. and W.T. Morgen, 1993, Cell Immunol. 152: 544) . Human HPRG immobilized at 1 μg/ml (100 μl/well) will induce >65% MOLT4 cell adhesion (1 x 10E5 cells/well) in the presence of 7.5 μg/ml Concanvalin A.
Protein Name
Background Synonyms: HPRG,HRGP,THPH11
Molecular Weight 59 kDa, 75-80 kDa
NCBI Accession NP_000403
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months