Pyruvate Kinase, Liver and RBC (PKLR) (AA 47-574) protein (His tag)

Details for Product No. ABIN3216416
Protein Name
Protein Characteristics
AA 47-574
6
2
1
1
1
1
1
Origin
Human
13
3
2
1
1
Source
Escherichia coli (E. coli)
10
2
2
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
His tag
Application
SDS-PAGE (SDS)
Options
Characteristics The recombinant human PKLR consists of 546 amino acids and predicts a molecular mass of 59.1 KDa. It migrates as an approximately 53-60 KDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human PKLR (NP_000289.1) (Leu47-Ser574) was expressed with a polyhistidine tag at the N-terminus.
Predicted N-Term: His
Purity > 85 % as determined by SDS-PAGE
Biological Activity Comment Kinase activity untested
Protein Name
Background Synonyms: PK1,PKL,PKR,PKRL,RPK
NCBI Accession NP_000289
Pathways Ribonucleoside Biosynthetic Process
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Expiry Date 12 months