Doublecortin-Like Kinase 1 (DCLK1) (AA 1-705) Protein

Details for Product No. ABIN3216427
Protein Name
Protein Characteristics
AA 1-705
1
1
1
1
1
1
1
1
1
1
Origin
Human
8
2
1
1
Source
Baculovirus infected Insect Cells
4
3
2
1
1
1
Protein Type
Recombinant
Application
SDS-PAGE (SDS)
Options
Characteristics The secreted recombinant human DCLK1 consists of 707 amino acids and predicts a molecular mass of 78.5 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Structure: A DNA sequence encoding the human DCLK1 ( O15075-1) (Met 1-Val 705) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Predicted N-Term: Gly
Purity > 85 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Kinase activity untested
Protein Name
Background Synonyms: CL1,CLICK1,DCAMKL1,DCDC3A,DCLK
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Expiry Date 12 months