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IgE Protein

Origin: Human Host: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, IsoC, ELISA, Func, FACS
Catalog No. ABIN363697
  • Target See all IgE Proteins
    IgE
    Protein Type
    Recombinant
    Origin
    • 8
    • 7
    • 4
    • 4
    • 2
    Human
    Source
    • 9
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    Human
    Application
    Western Blotting (WB), Isotype Control (IsoC), ELISA, Functional Studies (Func), Flow Cytometry (FACS)
    Specificity
    Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown
    Characteristics
    IGHE, chromosome 14q32.33
    Source of protein: Human B cell hybridoma from healthy donors (CD40 cell culture system)
    Purification
    Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
    Purity
    > 95 %, tested by analytical Gel Permeation Chromatography
    Top Product
    Discover our top product IgE Protein
  • Application Notes
    For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS, pH=7.2, 0.1 % NaN3
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Storage
    4 °C
  • Target
    IgE
    Abstract
    IgE Products
    Synonyms
    IGH2 Protein, IgE Protein, Gm900 Protein, immunoglobulin heavy chain (epsilon polypeptide) Protein, immunoglobulin heavy constant epsilon Protein, Immunoglobulin heavy constant epsilon Protein, Ighe Protein, IGHE Protein
    Background
    Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs mAb LE27 or a specific recombinant cross-linking agent.
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