Calpain 1 (CAPN1) (Active) Protein

Details for Product No. ABIN411905, Supplier: Log in to see
Protein Name
  • CAPN1
  • Capa-1
  • Capa1
  • mu-calpin
  • Capn1
  • fj67g01
  • wu:fj67g01
  • calpain 1
  • zgc:55262
  • CAPN1
  • Capn1
  • zgc:55262
Origin
Human
4
1
Source
Human
3
1
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
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Protein Source Erythrocyte
Purification Prepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV 1 and 2, HBc and HCV by FDA required tests.
Purity ≥95 % by SDS-PAGE
Biological Activity Comment Specification / Test Method: >1000 units/mg protein
Unit Definition One unit is defined as the amount of enzyme that will hydrolyze one pmol Suc-LLVY-AMC per minute at 25 °C.
Background Ca²ᶧ,-dependent cysteine proteinase with low Ca²ᶧ,requirement (half maximal activation = 2 μM). Participates in the ATP release reaction of platelets stimulated with thrombin. Involves in apoptosis activation.

Alternative Names: Calcium-activated neutral proteinase 2, Short name=CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, Short name=M-calpain
Molecular Weight 112.0 kDa
Gene ID 823
UniProt P07384
Research Area Proteolysis / Ubiquitin
Application Notes The protein concentration is determined by the Lowry Method using BSA as a standard.
Optimal working dilution should be determined by the investigator.
Protocol 1. Incubate the reaction mixture at 30 °C for 30 minutes.
2. Stop the reaction by adding 1 mL of 5 % TCA.
3. Centrifuge at 10,000x g for 10 minutes.
4. Transfer 0.4 mL of the supernatant into a test tube.
5. Add 0.1 mL of 0.7 M Na 2 CO 3 (in 0.1 N NaOH).
6. Add 0.1 mL of 2 M Iodoacetic acid (in 2 N NaOH).
7. Add 2.0 mL of freshly prepared mixture of 40 µL 0.5 % CuSO 4, 40 µL 1 % Sodium-Potassium Tartarate, and 1.92 mL of 1.5 % Na2CO3.
8. Mix thoroughly and let stand for 10 minutes at room temperature.
9. Add 0.2 mL of 1 N Folin-Phenol reagent. Mix quickly by vortex.
10. Let stand for 30 minutes at room temperature.
11. Measure absorbance at 750 nm (A 750nm ).
Restrictions For Research Use only
Format Liquid
Buffer In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30 % glycerol, pH 6.8.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Expiry Date 12 months
Product cited in: Wang, Zhang, Li, Tang, Siedlak, Fujioka, Liu, Su, Pi, Wang: "MFN2 couples glutamate excitotoxicity and mitochondrial dysfunction in motor neurons." in: The Journal of biological chemistry, Vol. 290, Issue 1, pp. 168-82, 2015 (PubMed).

Chen, Yao, Hasegawa, Akamatsu, Yoshimura, Hosoi: "Effects of isoproterenol on aquaporin 5 levels in the parotid gland of mice in vivo." in: American journal of physiology. Endocrinology and metabolism, Vol. 306, Issue 1, pp. E100-8, 2014 (PubMed).

Müller, Freed, Dhalla: "Activation of proteases and changes in Na+-K+-ATPase subunits in hearts subjected to ischemia-reperfusion." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 114, Issue 3, pp. 351-60, 2013 (PubMed).

Mitchell, Samulski: "Mechanistic insights into the enhancement of adeno-associated virus transduction by proteasome inhibitors." in: Journal of virology, Vol. 87, Issue 23, pp. 13035-41, 2013 (PubMed).

Huang, Zhou, Saberwal, Konieczna, Horvath, Katsoulidis, Platanias, Eklund: "Interferon consensus sequence binding protein (ICSBP) decreases beta-catenin activity in myeloid cells by repressing GAS2 transcription." in: Molecular and cellular biology, Vol. 30, Issue 19, pp. 4575-94, 2010 (PubMed).

Murakami, Tolstykh, Bao, Niu, Steuer, Bao, Chang, Espiritu, Bansal, Lam, Otto, Sofia, Furman: "Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34337-47, 2010 (PubMed).

Saraswathy, Rao: "Mitochondrial proteomics in experimental autoimmune uveitis oxidative stress." in: Investigative ophthalmology & visual science, Vol. 50, Issue 12, pp. 5559-66, 2009 (PubMed).