Pregnancy Up-Regulated Non-Ubiquitously Expressed CaM Kinase (PNCK) (Active) Protein

Details for Product No. ABIN411936, Supplier: Log in to see
Protein Name
  • BSTK3
  • CaMK1b
  • Bstk3
  • CaMKIbeta2
  • CaMKlb2
  • Camk1b
  • Punc
  • caMKlb1
  • pregnancy up-regulated nonubiquitous CaM kinase
  • pregnancy upregulated non-ubiquitously expressed CaM kinase
  • PNCK
  • Pnck
Origin
Human
5
2
1
Source
Baculovirus infected Insect Cells
3
1
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >90% Pure active Recombinant CAMK1b.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background Many of the effects of calcium are mediated via its interaction with calmodulin and the subsequent activation of Ca(2+)/calmodulin-dependent (CaM) kinases. CaM kinases are involved in a wide variety of cellular processes including muscle contraction, neurotransmitter release, cell cycle control, and transcriptional regulation. While CaMKII has been implicated in learning and memory, the biological role of the other multifunctional CaM kinases, CaMKI and CaMKIV, is largely unknown. CaMKIβ, or pregnancy upregulated non-ubiquitously expressed CaM kinase (PNCK), is a 38- kDa serine/threonine kinase whose catalytic domain shares 45-70 % identity with members of the CaM kinase family. The gene for CaMKIβ localizes to mouse chromosome X. CaMKIβ is upregulated during intermediate and late stages of murine fetal development with highest levels of expression in developing brain, bone, and gut. CaMKIβ is also expressed in a tissue-specific manner in adult mice with highest levels of expression detected in brain, uterus, ovary, and testis. Interestingly, CaMKIβ expression in these tissues is restricted to particular compartments and appears to be further restricted to subsets of cells within those compartments. The chromosomal localization of CaMKIβ, along with its tissue-specific and restricted pattern of spatial expression during development, suggests that CaMKIβ may be involved in a variety of developmental processes including development of the central nervous system (1). Also CaMKIβ2, an isoform of mCaMKIβ, was mainly identified in the nervous system, including brain, spinal cord, trigeminal ganglion, and retina. Within the CNS, the expression of CaMKIβ2 is detected in the mantle zone, but not in the ventricular zone, suggesting its possible involvement in the differentiation of neurons (2).
Alternate Names: CAMK1b, Calcium/calmodulin-dependent protein kinase type I beta chain
Molecular Weight 64.0 kDa
Gene ID 8536
NCBI Accession NM_012040
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 223 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
Activity Assay (AcA) image for Pregnancy Up-Regulated Non-Ubiquitously Expressed CaM Kinase (PNCK) (Active) protein (ABIN411936) Pregnancy Up-Regulated Non-Ubiquitously Expressed CaM Kinase (PNCK) (Active) protein
SDS-PAGE (SDS) image for Pregnancy Up-Regulated Non-Ubiquitously Expressed CaM Kinase (PNCK) (Active) protein (ABIN411936) Pregnancy Up-Regulated Non-Ubiquitously Expressed CaM Kinase (PNCK) (Active) protein (Image 2)
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