WARS Protein (AA 1-471) (His tag)

Catalog No. ABIN4370383

Product Details

Target: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Protein Type: Recombinant

Protein Characteristics: AA 1-471

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This WARS protein is labelled with His tag.

Purpose: Recombinant Human Tryptophan--tRNA Ligase, Cytoplasmic/WARS/TrpRS (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MPNSEPASLL ELFNSIATQG ELVRSLKAGN ASKDEIDSAV KMLVSLKMSY KAAAGEDYKA DCPPGNPAPT SNHGPDATEA EEDFVDPWTV QTSSAKGIDY DKLIVRFGSS KIDKELINRI ERATGQRPHH FLRRGIFFSH RDMNQVLDAY ENKKPFYLYT GRGPSSEAMH VGHLIPFIFT KWLQDVFNVP LVIQMTDDEK YLWKDLTLDQ AYSYAVENAK DIIACGFDIN KTFIFSDLDY MGMSSGFYKN VVKIQKHVTF NQVKGIFGFT DSDCIGKISF PAIQAAPSFS NSFPQIFRDR TDIQCLIPCA IDQDPYFRMT RDVAPRIGYP KPALLHSTFF PALQGAQTKM SASDPNSSIF LTDTAKQIKT KVNKHAFSGG RDTIEEHRQF GGNCDVDVSF MYLTFFLEDD DKLEQIRKDY TSGAMLTGEL KKALIEVLQP LIAEHQARRK EVTDEIVKEF MTPRKLSFDF Q

Characteristics: Recombinant HumanTryptophan--tRNA ligase, cytoplasmic/WARS is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Gln471) of Human WARS fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 6 months

Target Details

Target: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Alternative Name: WARS (WARS Products)

Synonyms: GAMMA-2 Protein, IFI53 Protein, IFP53 Protein, zgc:73274 Protein, wars Protein, MGC53671 Protein, WARS Protein, TrpRS Protein, WRS Protein, 85D-WRS Protein, CG9735 Protein, Dmel\\CG9735 Protein, WRS-85D Protein, l(3)03559 Protein, l(3)03560 Protein, l(3)3559 Protein, gamma-2 Protein, ifi53 Protein, ifp53 Protein, GB14934 Protein, ECK3371 Protein, JW3347 Protein, tryptophanyl-tRNA synthetase Protein, tryptophanyl-tRNA synthetase S homeolog Protein, Tryptophanyl-tRNA synthetase Protein, tryptophanyl-tRNA synthetase L homeolog Protein, tryptophan--tRNA ligase, cytoplasmic Protein, WARS Protein, wars Protein, wars.S Protein, Wars Protein, TrpRS Protein, wars.L Protein, LOC727582 Protein, trpS Protein, trpS2 Protein

Sub Type: Fusionprotein

Background: There exists two types of tryptophanyl tRNA synthetases, the cytoplasmic form called WARS, the mitochondrial form called WARS2. WARS catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. WARS regulates ERK, Akt, eNOS activation pathway, which are related with angiogenesis, cytoskelatal reorganization and shear stess-reponsive gene expression.
Alternative Names: WARS also known as Tryptophanyl-tRNA synthetase, Interferon-induced protein 53.

Molecular Weight: 55.3 kDa

UniProt: P23381