MASP1 Protein (AA 20-728) (His tag)

Catalog No. ABIN4370440

Product Details

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Protein Type: Recombinant

Protein Characteristics: AA 20-728

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This MASP1 protein is labelled with His tag.

Purpose: Recombinant Human Mannan-Binding Lectin Serine Protease 1/MASP1 (C-6His)

Sequence: HTVELNNMFG QIQSPGYPDS YPSDSEVTWN ITVPDGFRIK LYFMHFNLES SYLCEYDYVK VETEDQVLAT FCGRETTDTE QTPGQEVVLS PGSFMSITFR SDFSNEERFT GFDAHYMAVD VDECKEREDE ELSCDHYCHN YIGGYYCSCR FGYILHTDNR TCRVECSDNL FTQRTGVITS PDFPNPYPKS SECLYTIELE EGFMVNLQFE DIFDIEDHPE VPCPYDYIKI KVGPKVLGPF CGEKAPEPIS TQSHSVLILF HSDNSGENRG WRLSYRAAGN ECPELQPPVH GKIEPSQAKY FFKDQVLVSC DTGYKVLKDN VEMDTFQIEC LKDGTWSNKI PTCKIVDCRA PGELEHGLIT FSTRNNLTTY KSEIKYSCQE PYYKMLNNNT GIYTCSAQGV WMNKVLGRSL PTCLPECGQP SRSLPSLVKR IIGGRNAEPG LFPWQALIVV EDTSRVPNDK WFGSGALLSA SWILTAAHVL RSQRRDTTVI PVSKEHVTVY LGLHDVRDKS GAVNSSAARV VLHPDFNIQN YNHDIALVQL QEPVPLGPHV MPVCLPRLEP EGPAPHMLGL VAGWGISNPN VTVDEIISSG TRTLSDVLQY VKLPVVPHAE CKTSYESRSG NYSVTENMFC AGYYEGGKDT CLGDSGGAFV IFDDLSQRWV VQGLVSWGGP EECGSKQVYG VYTKVSNYVD WVWEQMGLPQ SVVEPQVERV DHHHHHH

Characteristics: Recombinant Human Mannan-Binding Lectin Serine Protease 1/MASP1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (His20-Arg728) of Human MASP1 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,200 mM NaCl,10 % Glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Alternative Name: MASP1 (MASP1 Products)

Synonyms: AW048060 Protein, CCPII Protein, Crarf Protein, Masp1/3 Protein, 3MC1 Protein, CRARF Protein, CRARF1 Protein, MAP1 Protein, MASP Protein, MASP3 Protein, MAp44 Protein, PRSS5 Protein, RaRF Protein, mannan-binding lectin serine peptidase 1 Protein, mannan binding lectin serine peptidase 1 Protein, Masp1 Protein, MASP1 Protein

Sub Type: Fusionprotein

Background: Mannan-Binding Lectin Serine Protease 1 (MASP-1) belongs to the peptidase S1 family. MASP1 contains two CUB domains, one EGF-like domain, one peptidase S1 domain and two Sushi (CCP/SCR) domains. MASP1 is primarily expressed in liver. MASP1 involved in the lectin pathway of the complement, performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. MASP1 is synthesized as a zymogen and activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. MASP1 is not directly involved in complement activation but may act as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP2. MASP1 is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. MASP1 is inhibited by SERPING1 and A2M.
Alternative Names: Mannan-Binding Lectin Serine Protease 1, Complement Factor MASP-3, Complement-Activating Component of Ra-Reactive Factor, Mannose-Binding Lectin-Associated Serine Protease 1, MASP-1, Mannose-Binding Protein-Associated Serine Protease, Ra-Reactive Factor S

Molecular Weight: 80.68 kDa

UniProt: P48740

Pathways: Complement System