CD73 Protein (AA 29-550) (His tag)
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- Target See all CD73 (NT5E) Proteins
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-550
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Origin
- Mouse
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Source
- Human Cells
- Purification tag / Conjugate
- This CD73 protein is labelled with His tag.
- Purpose
- Recombinant Mouse 5'-Nucleotidase/NT5E/CD73 (C-6His)
- Sequence
- WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVQAYAFGKY LGYLKVEFDD KGNVITSYGN PILLNSSIPE DATIKADINQ WRIKLDNYST QELGRTIVYL DGSTQTCRFR ECNMGNLICD AMINNNLRHP DEMFWNHVSM CIVNGGGIRS PIDEKNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDINRKPW NRVVQLEVLC TKCRVPIYEP LEMDKVYKVT LPSYLANGGD GFQMIKDELL KHDSGDQDIS VVSEYISKMK VVYPAVEGRI KFHHHHHH
- Characteristics
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Recombinant Mouse 5'-Nucleotidase/NT5E/CD73 (C-6His)
Specific Activity is greater than 34000pmol/min/ugMeasured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product NT5E Protein
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5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-549) protein (His tag)
NT5E Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) protein (His tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) protein (Fc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-554) protein (His tag)NT5E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (His tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (Fc Tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) protein (His tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) (Active) protein (His tag,AVI tag,Biotin)NT5E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 34-214) protein (His tag)NT5E Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 28-264) protein (GST tag)NT5E Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 120 mM NaCl,4 mM CaCl2, 20 % Glycerol, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Alternative Name
- 5'-Nucleotidase (NT5E Products)
- Synonyms
- CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein
- Molecular Weight
- 58.8 kDa
- UniProt
- Q61503
- Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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