NPPB Protein (AA 27-134) (His tag)

Catalog No. ABIN7313048

Product Details

Target: NPPB (Natriuretic Peptides B (NPPB))

Protein Type: Recombinant

Protein Characteristics: AA 27-134

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NPPB protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purpose: Recombinant Human NPPB Protein is produced by our E. coli expression system and the target gene encoding His27-His134 is expressed with a 6His tag at the N-terminus.

Sequence: MGSSHHHHHH SSGLVPRGSH MHPLGSPGSA SDLETSGLQE QRNHLQGKLS ELQVEQTSLE PLQESPRPTG VWKSREVATE GIRGHRKMVL YTLRAPRSPK MVQGSGCFGR KMDRISSSSG LGCKVLRRH

Purity: Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 20 % Glycerol, pH 7.4

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: NPPB (Natriuretic Peptides B (NPPB))

Alternative Name: NPPB (NPPB Products)

Synonyms: BNP1 Protein, NPPB Protein, BNP Protein, Bnf Protein, AA408272 Protein, natriuretic peptide B Protein, natriuretic peptide type B Protein, NPPB Protein, Nppb Protein

Gene ID: 4879

UniProt: P16860

Pathways: Hormone Activity