PKLR Protein (AA 1-574) (His tag)
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- Target See all PKLR Proteins
- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-574
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This PKLR protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human PKLR Protein is produced by our mammalian expression system and the target gene encoding Met1-Ser574 is expressed with a 6His tag at the C-terminus.
- Sequence
- MSIQENISSL QLRSWVSKSQ RDLAKSILIG APGGPAGYLR RASVAQLTQE LGTAFFQQQQ LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS VERLKEMIKA GMNIARLNFS HGSHEYHAES IANVREAVES FAGSPLSYRP VAIALDTKGP EIRTGILQGG PESEVELVKG SQVLVTVDPA FRTRGNANTV WVDYPNIVRV VPVGGRIYID DGLISLVVQK IGPEGLVTQV ENGGVLGSRK GVNLPGAQVD LPGLSEQDVR DLRFGVEHGV DIVFASFVRK ASDVAAVRAA LGPEGHGIKI ISKIENHEGV KRFDEILEVS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NLAGKPVVCA TQMLESMITK PRPTRAETSD VANAVLDGAD CIMLSGETAK GNFPVEAVKM QHAIAREAEA AVYHRQLFEE LRRAAPLSRD PTEVTAIGAV EAAFKCCAAA IIVLTTTGRS AQLLSRYRPR AAVIAVTRSA QAARQVHLCR GVFPLLYREP PEAIWADDVD RRVQFGIESG KLRGFLRVGD LVIVVTGWRP GSGYTNIMRV LSISVDHHHH HH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product PKLR Protein
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Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (GST tag)
PKLR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 485-574) protein (GST tag)PKLR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 47-574) protein (His tag)PKLR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (Strep Tag)Crystallography grade PKLR Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (Strep Tag)Crystallography grade PKLR Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 1 mM DTT, 0.2M NaCl, 10 % glycerol, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
- Alternative Name
- PKLR (PKLR Products)
- Synonyms
- PK1 Protein, PKL Protein, PKR Protein, PKRL Protein, RPK Protein, Pklg Protein, wu:fd15e01 Protein, wu:fi37e08 Protein, pk1 Protein, PKLR Protein, Pk-1 Protein, Pk1 Protein, R-PK Protein, pklr Protein, pyruvate kinase L/R Protein, pyruvate kinase, liver and RBC Protein, pyruvate kinase, liver and RBC L homeolog Protein, pyruvate kinase liver and red blood cell Protein, pyruvate kinase PKLR-like Protein, PKLR Protein, Pklr Protein, pklr Protein, pklr.L Protein, LOC100621940 Protein
- Gene ID
- 5313
- UniProt
- P30613
- Pathways
- Ribonucleoside Biosynthetic Process
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