UBE2D4 Protein (AA 1-147) (GST tag)

Catalog No. ABIN7313896

Product Details

Target: UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))

Protein Type: Recombinant

Protein Characteristics: AA 1-147

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2D4 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Mass Spectrometry (MS)

Purpose: Recombinant Human UBE2D4 Protein is produced by our E.coli expression system and the target gene encoding Met1-Met147 is expressed with a GST tag at the N-terminus.

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFH MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM

Purity: Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μM filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))

Alternative Name: UBE2D4 (UBE2D4 Products)

Synonyms: HBUCE1 Protein, UBE2D4 Protein, RGD69425 Protein, Ube2d2 Protein, Ube2d2b Protein, zgc:162263 Protein, ube2d3.2 Protein, EG546638 Protein, Gm5959 Protein, ubiquitin conjugating enzyme E2 D4 (putative) Protein, ubiquitin-conjugating enzyme E2D 4 (putative) Protein, ubiquitin-conjugating enzyme E2 D4 Protein, ubiquitin conjugating enzyme E2D 4 (putative) L homeolog Protein, ubiquitin-conjugating enzyme E2 D2 pseudogene Protein, ubiquitin-conjugating enzyme E2D 4 Protein, UBE2D4 Protein, Ube2d4 Protein, EDI_085450 Protein, EDI_185830 Protein, ube2d4 Protein, ube2d4.L Protein, LOC100354069 Protein

Gene ID: 51619

UniProt: Q9Y2X8