Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-387) protein (His tag)

Details for Product No. ABIN4888095, Supplier: Log in to see
Protein Name
  • CG10523
  • Dmel\\CG10523
  • Dpark
  • SD01679
  • dpk
  • AR-JP
  • LPRS2
  • PDJ
  • PRKN
  • Park
  • Prkn
  • si:ch211-123f21.1
  • zgc:112390
  • pdr-1
  • PARK2
  • parkin
  • parkin RBR E3 ubiquitin protein ligase
  • Parkinson disease (autosomal recessive, juvenile) 2, parkin
  • park
  • PRKN
  • Prkn
  • prkn
  • CpipJ_CPIJ014867
  • Park2
Protein Characteristics
AA 1-387
3
2
1
1
1
1
1
Origin
Human
9
1
1
Source
Escherichia coli (E. coli)
4
2
2
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This PARK2 protein is labelled with His tag.
Application
ELISA, Mass Spectrometry (MS), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose Recombinant Human PARK2 Protein is produced by our E. coli expression system and the target gene encoding Met1-Lys387 is expressed with a 6His tag at the C-terminus.
Sequence MIVFVRFNSS HGFPVEVDSD TSIFQLKEVV AKRQGVPADQ LRVIFAGKEL RNDWTVQNCD LDQQSIVHIV QRPWRKGQEM NATGGDDPRN AAGGCEREPQ SLTRVDLSSS VLPGDSVGLA VILHTDSRKD SPPAGSPAGR SIYNSFYVYC KGPCQRVQPG KLRVQCSTCR QATLTLTQGP SCWDDVLIPN RMSGECQSPH CPGTSAEFFF KCGAHPTSDK ETSVALHLIA TNSRNITCIT CTDVRSPVLV FQCNSRHVIC LDCFHLYCVT RLNDRQFVHD PQLGYSLPCV GTGDTVVLRG ALGGFRRGVA GCPNSLIKEL HHFRILGEEQ YNRYQQYGAE ECVLQMGGVL CPRPGCGAGL LPEPDQRKVT CEGGNGLGCG YGQRRTKLEH HHHHH
Purity Greater than 95 % as determined by reducing SDS-PAGE.
Sterility 0.2 μm filtered
Endotoxin Level Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Gene ID 5071
UniProt O60260
Restrictions For Research Use only
Format Liquid
Buffer Supplied as a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, 2 mM DTT, 20 % Glycerol, pH 7.4.
Handling Advice Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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