Tumor Necrosis Factor (TNF) (Active) Protein

Details for Product No. ABIN4889933, Supplier: Log in to see
Protein Name
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LOC103694380
  • tnfa
Origin
Human
119
61
35
9
7
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
181
28
20
6
3
3
2
2
1
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
ELISA, In vitro Assay (in vitro), In vivo Studies (in vivo), Western Blotting (WB)
Options
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Sequence MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Purity ≥ 95%
Sterility 0.2 μm filtered
Endotoxin Level ≤ 1.00 LAL (EUs/ug)
Biological Activity Comment
  • Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D
  • Bioactivity Acceptance Criteria ED50 (ng/ml): 2 ng/mL
  • Bioactivity Acceptance Criteria (Units/mg): 5 x 10^5 units/mg
Protein Name
Background Tumor necrosis factor alpha (TNFa) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNFa signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNFa functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

Alternate Names: TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF
Molecular Weight Monomer: 17.5 MW Monomer/Total (kDa)
UniProt P01375
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Media Components: Luria Broth

Restrictions For Research Use only
Format Lyophilized
Reconstitution Sterile water at 0.1 mg/mL

Store at 4 °C and avoid freezing.
Buffer Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing : 10 mM sodium phosphate,  pH 7.5
Preservative Without preservative
Handling Advice Centrifuge vial before opening.
Storage 4 °C,-20 °C,-80 °C
Storage Comment 12 months from date of receipt when stored at -20°C to-80°C as supplied. 1 month when stored at 4°C after reconstituting as directed.
Expiry Date 12 months
Supplier Images
 image for Tumor Necrosis Factor (TNF) (Active) protein (ABIN4889933) Tumor Necrosis Factor (TNF) (Active) protein
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