Interleukin 10 (IL10) (Active) Protein

Details for Product No. ABIN4889937, Supplier: Log in to see
Protein Name
  • CSIF
  • GVHDS
  • IL-10
  • IL10A
  • TGIF
  • Il-10
  • IL10X
  • interleukin 10
  • IL10
  • Il10
Origin
Mouse (Murine)
87
39
23
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
119
26
21
12
3
2
1
Protein Type
Recombinant
Biological Activity
Active
Application
ELISA, In vitro Assay (in vitro), In vivo Studies (in vivo), Western Blotting (WB)
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Sequence MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGEKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLQDQGVYKA MNEFDIFINC IEAYMMIKMK S
Purity ≥ 95%
Sterility 0.2 μm filtered
Endotoxin Level ≤ 1.00 LAL (EUs/ug)
Biological Activity Comment
  • Bioactivity Assay: MC/9 cell proliferation
  • Bioactivity Acceptance Criteria ED50 (ng/ml): 5 ng/mL
  • Bioactivity Acceptance Criteria (Units/mg): 2.0 x 10^5 units/mg
Protein Name
Background Interleukin 10 (IL-10) is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells. IL-10 inhibits the production of pro-inflammatory cytokines such as interferon gamma (IFNgamma), tumor necrosis factor alpha (TNFα), interleukin 2 (IL-2), interleukin 3 (IL-3), interleukin 4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF), made by macrophages and regulatory T cells. IL-10 also suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells. Mouse IL-10 is not active on human cells.

Alternate Names: Cytokine synthesis inhibitory factor, CSIF
Molecular Weight Non-Covalent Homodimer: 18.9 MW Monomer/Total (kDa)
UniProt P18893
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Media Components: Luria Broth

Restrictions For Research Use only
Format Lyophilized
Reconstitution Sterile water at 0.1 mg/mL
Buffer Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing : 10 mM sodium phosphate,  pH 7.5
Preservative Without preservative
Handling Advice Centrifuge vial before opening.
Storage 4 °C,-20 °C,-80 °C
Storage Comment 12 months from date of receipt when stored at -20°C to-80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.
Expiry Date 12 months
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