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VTCN1 Protein (AA 33-194) (His tag,AVI tag,Biotin)

VTCN1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func
Catalog No. ABIN4949005
  • Target See all VTCN1 Proteins
    VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 33-194
    Origin
    • 26
    • 6
    • 6
    • 3
    • 2
    • 1
    Human
    Source
    • 26
    • 6
    • 6
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This VTCN1 protein is labelled with His tag,AVI tag,Biotin.
    Application
    Functional Studies (Func)
    Brand
    MABSol®,PrecisionAvi
    Sequence
    AA 33-194
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 20.8 kDa. As a result of glycosylation, the protein migrates as 35-45 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).
    Purity
    >95 % as determined by SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product VTCN1 Protein
  • Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
  • Johnston, Su, Pinckney, Critton, Boyer, Krishnakumar, Corbett, Rankin, Dibella, Campbell, Martin, Lemar, Cayton, Huang, Deng, Nayeem, Chen, Ergel, Rizzo, Yamniuk, Dutta, Ngo, Shorts, Ramakrishnan et al.: "VISTA is an acidic pH-selective ligand for PSGL-1. ..." in: Nature, Vol. 574, Issue 7779, pp. 565-570, (2019) (PubMed).

  • Target
    VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
    Alternative Name
    B7-H5 (VTCN1 Products)
    Synonyms
    B7h4 Protein, B7s1 Protein, B7x Protein, BC032925 Protein, B7-H4 Protein, B7H4 Protein, B7S1 Protein, B7X Protein, B7h.5 Protein, PRO1291 Protein, VCTN1 Protein, RGD1311204 Protein, V set domain containing T cell activation inhibitor 1 L homeolog Protein, V-set domain containing T cell activation inhibitor 1 Protein, V-set domain containing T-cell activation inhibitor 1 Protein, V set domain containing T cell activation inhibitor 1 Protein, vtcn1.L Protein, VTCN1 Protein, vtcn1 Protein, Vtcn1 Protein
    Background
    Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
    Molecular Weight
    20.8 kDa
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