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BTLA Protein (AA 31-134) (Fc Tag,AVI tag,Biotin)

BTLA Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Catalog No. ABIN4949010
  • Target See all BTLA Proteins
    BTLA (B and T Lymphocyte Associated (BTLA))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 31-134
    Origin
    • 32
    • 12
    • 2
    • 1
    Human
    Source
    • 28
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This BTLA protein is labelled with Fc Tag,AVI tag,Biotin.
    Application
    Functional Studies (Func)
    Brand
    MABSol®,PrecisionAvi
    Sequence
    AA 31-134
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    Purity
    >95 % as determined by SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
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    Discover our top product BTLA Protein
  • Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    Tris with Glycine, Arginine and NaCl,  pH 7.5
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
  • Target
    BTLA (B and T Lymphocyte Associated (BTLA))
    Alternative Name
    BTLA (BTLA Products)
    Synonyms
    BTLA Protein, BTLA1 Protein, CD272 Protein, A630002H24 Protein, B and T lymphocyte associated Protein, BTLA Protein, Btla Protein
    Background
    B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
    Molecular Weight
    40.5 kDa
    UniProt
    Q7Z6A9
    Pathways
    Cancer Immune Checkpoints
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