IL17RA Protein (AA 33-320) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN4949028

Product Details

Target: IL17RA (Interleukin 17 Receptor A (IL17RA))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 33-320

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL17RA protein is labelled with Fc Tag,AVI tag,Biotin.

Application: Functional Studies (Func)

Brand: MABSol®,PrecisionAvi

Sequence: AA 33-320

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 61.9 kDa. As a result of glycosylation, the protein migrates as 80-115 kDa under reducing (R) condition, and 150-200 kDa under non-reducing (NR) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: IL17RA (Interleukin 17 Receptor A (IL17RA))

Alternative Name: IL-17 RA (IL17RA Products)

Synonyms: IL17R Protein, CANDF5 Protein, CD217 Protein, CDw217 Protein, IL-17RA Protein, hIL-17R Protein, Il17r Protein, AW538159 Protein, Cdw217 Protein, VDw217 Protein, interleukin 17 receptor A Protein, IL17RA Protein, Il17ra Protein

Background: Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5).

Molecular Weight: 61.9 kDa

NCBI Accession: NP_055154

Pathways: SARS-CoV-2 Protein Interactome