HVEM Protein (AA 39-202) (His tag)

Catalog No. ABIN4949111

Product Details

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 39-202

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This HVEM protein is labelled with His tag.

Application: Functional Studies (Func)

Sequence: AA 39-202

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.2 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >90 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

References

Szymczak, Ziętkiewicz, Kuncewicz, Rodziewicz-Motowidło, Orlikowska: "Expression, purification, and efficient refolding of the extracellular domain of Escherichia coli-expressed signaling receptor herpesvirus entry mediator." in: Protein expression and purification, Vol. 164, pp. 105450, (2019) (PubMed).

Target Details

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Alternative Name: HVEM (TNFRSF14 Products)

Synonyms: ATAR Protein, CD270 Protein, HVEA Protein, HVEM Protein, LIGHTR Protein, TR2 Protein, Atar Protein, HveA Protein, Hvem Protein, Tnfrs14 Protein, TNF receptor superfamily member 14 Protein, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Protein, Tnfrsf14 Protein, TNFRSF14 Protein

Target Type: Viral Protein

Background: Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

Molecular Weight: 19.2 kDa

NCBI Accession: NP_003811

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints