CD40 Ligand Protein (CD40LG) (AA 115-260) (His tag)

Catalog No. ABIN4949165

Product Details

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 115-260

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CD40 Ligand protein is labelled with His tag.

Application: Functional Studies (Func)

Sequence: AA 115-260

Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 21-25 kDa under reducing (R) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40 Ligand (CD40LG Products)

Synonyms: CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein

Background: CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.

Molecular Weight: 16.8 kDa

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints