UBE2K Protein (AA 2-200) (His tag)

Catalog No. ABIN4975966

Product Details

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Protein Type: Recombinant

Protein Characteristics: AA 2-200

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2K protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: ANIAVQRIKR EFKEVLKSEE TSKNQIKVDL VDENFTELRG EIAGPPDTPY EGGRYQLEIK IPETYPFNPP KVRFITKIWH PNISSVTGAI CLDILKDQWA AAMTLRTVLL SLQALLAAAE PDDPQDAVVA NQYKQNPEMF KQTARLWAHV YAGAPVSSPE YTKKIENLCA MGFDRNAVIV ALSSKSWDVE TATELLLSN

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: UBE2K (UBE2K Products)

Synonyms: E2-25K Protein, HIP2 Protein, HYPG Protein, LIG Protein, UBC1 Protein, E2-25k Protein, Hip2 Protein, Hypg Protein, Lig Protein, e2-25k Protein, hip2 Protein, hypg Protein, lig Protein, AW492011 Protein, D5Ertd601e Protein, ube2k Protein, zgc:103472 Protein, zgc:110791 Protein, ubiquitin conjugating enzyme E2 K Protein, ubiquitin-conjugating enzyme E2K Protein, ubiquitin conjugating enzyme E2 K S homeolog Protein, ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) Protein, ubiquitin-conjugating enzyme E2Ka (UBC1 homolog, yeast) Protein, UBE2K Protein, Ube2k Protein, ube2k.S Protein, ube2k Protein, ube2kb Protein, ube2ka Protein

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B, in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1

Molecular Weight: 26.4 kDa

UniProt: P61086