ISG15 Protein (AA 2-157) (His tag)
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- Target See all ISG15 Proteins
- ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-157
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ISG15 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- GWDLTVKMLA GNEFQVSLSS SMSVSELKAQ ITQKIGVHAF QQRLAVHPSG VALQDRVPLA SQGLGPGSTV LLVVDKCDEP LSILVRNNKG RSSTYEVRLT QTVAHLKQQV SGLEGVQDDL FWLTFEGKPL EDQLPLGEYG LKPLSTVFMN LRLRGG
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product ISG15 Protein
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ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-157) protein (CaM)
ISG15 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 98 % by SDS - PAGE SDS
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-165) protein (GST tag)ISG15 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-100) protein (GST tag)ISG15 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-155) protein (His tag)Crystallography grade ISG15 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157), (full length) protein (GST tag)ISG15 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157), (full length) protein (His tag)ISG15 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))
- Alternative Name
- ISG15 (ISG15 Products)
- Synonyms
- G1P2 Protein, IFI15 Protein, IP17 Protein, UCRP Protein, hUCRP Protein, ISG15 Protein, g1p2 Protein, si:rp71-1c23.2 Protein, G1p2 Protein, IGI15 Protein, Irfp Protein, ISG17 Protein, ISG17/UCRP Protein, ISG15 ubiquitin-like modifier Protein, ISG15 Protein, isg15 Protein, Isg15 Protein
- Background
- Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, TRIM25, STAT5A, MAPK1/ERK2 and globin. Can also isgylate: DDX58/RIG-I which inhibits its function in antiviral signaling response and EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A and B virus, sindbis virus (SV) and herpes simplex type-1 (HHV-1). Plays a significant role in the control of neonatal Chikungunya virus (CHIKV) infection by acting as a putative immunomodulator of proinflammatory cytokines. Protects mice against the consequences of Chikungunya virus infection by downregulating the pathogenic cytokine response, often denoted as the cytokine storm. Plays a role in erythroid differentiation. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity
- Molecular Weight
- 21.1 kDa
- UniProt
- Q64339
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