ALAS1 Protein (His tag)

Catalog No. ABIN5505552

Product Details

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ALAS1 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Purified recombinant protein of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Purification: Purified

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Alternative Name: aminolevulinate, delta-, synthase 1 (ALAS1 Products)

Background: This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 70.4 kDa

NCBI Accession: NP_000679

Pathways: Regulation of Lipid Metabolism by PPARalpha