MRPL22 Protein (His tag)

Catalog No. ABIN5505722

Product Details

Target: MRPL22 (Mitochondrial Ribosomal Protein L22 (MRPL22))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MRPL22 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Purified recombinant protein of Human mitochondrial ribosomal protein L22 (MRPL22), nuclear gene encoding mitochondrial protein, transcript variant 2, full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag, transcript variant 2) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Purification: Purified

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MRPL22 (Mitochondrial Ribosomal Protein L22 (MRPL22))

Alternative Name: mitochondrial ribosomal protein L22 (MRPL22 Products)

Synonyms: L22mt Protein, MRP-L22 Protein, MRP-L25 Protein, RPML25 Protein, E030011D16Rik Protein, HSPC158 Protein, Rpml25 Protein, GB10944 Protein, DDBDRAFT_0205817 Protein, DDBDRAFT_0267022 Protein, DDB_0205817 Protein, DDB_0267022 Protein, zgc:171911 Protein, CG4742 Protein, Dmel\\CG4742 Protein, L22 Protein, mitochondrial ribosomal protein L22 Protein, 39S ribosomal protein L22, mitochondrial Protein, mitochondrial 54S ribosomal protein YmL22 Protein, ribosomal protein L22, mitochondrial Protein, mitochondrial ribosomal protein subunit L22 Protein, MRPL22 Protein, Mrpl22 Protein, LOC413121 Protein, mrpl22 Protein, CAALFM_C403410WA Protein, mRpL22 Protein

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 14.5 kDa

NCBI Accession: NP_001014990