NFYA Protein (His tag)
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- Target See all NFYA Proteins
- NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NFYA protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Purified recombinant protein of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 2, full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag, transcript variant 2) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purification
- Purified
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product NFYA Protein
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Nuclear Transcription Factor Y, alpha (NFYA) (AA 1-318) protein (His tag)
NFYA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nuclear Transcription Factor Y, alpha (NFYA) (AA 1-347) protein (GST tag)NFYA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nuclear Transcription Factor Y, alpha (NFYA) (AA 219-318) protein (GST tag)NFYA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nuclear Transcription Factor Y, alpha (NFYA) (AA 1-347) protein (Strep Tag)Crystallography grade NFYA Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Nuclear Transcription Factor Y, alpha (NFYA) (AA 1-346) protein (Strep Tag)Crystallography grade NFYA Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
- Alternative Name
- nuclear transcription factor Y, alpha (NFYA Products)
- Synonyms
- wu:fb16e03 Protein, ik:tdsubc_2b6 Protein, xx:tdsubc_2b6 Protein, NFYA Protein, CBF-A Protein, CBF-B Protein, HAP2 Protein, NF-YA Protein, AA407810 Protein, Cbf-b Protein, SEZ-10 Protein, SEZ10 Protein, nuclear transcription factor Y, alpha Protein, nuclear transcription factor Y subunit alpha Protein, nuclear transcription factor-Y alpha Protein, nfya Protein, NFYA Protein, Nfya Protein
- Background
- The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
- Molecular Weight
- 33.8 kDa
- NCBI Accession
- NP_068351
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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