RYK Protein (DYKDDDDK-His Tag)

Catalog No. ABIN5505758

Product Details

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This RYK protein is labelled with DYKDDDDK-His Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Purified recombinant protein of human receptor-like tyrosine kinase (RYK), transcript variant 1, with C-terminal DDK/His tag, expressed in human cells, 20 μg (C-term DDK/His tag, transcript variant 1) protein expressed in Human HEK293 cells.
• Produced with end-sequenced ORF clone

Purification: Purified

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 1 x PBS, pH 7.4, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Alternative Name: receptor-like tyrosine kinase (RYK Products)

Synonyms: RYK Protein, jtk5 Protein, ryk1 Protein, jtk5a Protein, d3s3195 Protein, wu:fb37b12 Protein, wu:fi05b06 Protein, zgc:158381 Protein, MGC114645 Protein, D3S3195 Protein, JTK5 Protein, JTK5A Protein, RYK1 Protein, AW536699 Protein, ERK-3 Protein, Vik Protein, receptor-like tyrosine kinase Protein, receptor-like tyrosine kinase S homeolog Protein, tyrosine-protein kinase RYK Protein, RYK Protein, ryk Protein, ryk.S Protein, LOC100541106 Protein, Ryk Protein

Background: The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 25 kDa

NCBI Accession: NP_001005861

Pathways: RTK Signaling, WNT Signaling, Regulation of Cell Size