SIRT3 Protein (His tag)
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- Target See all SIRT3 Proteins
- SIRT3 (Sirtuin 3 (SIRT3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SIRT3 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Purified recombinant protein of Human sirtuin 3 (SIRT3), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purification
- Purified
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product SIRT3 Protein
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Sirtuin 3 (SIRT3) (AA 1-399) protein (GST tag)
SIRT3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sirtuin 3 (SIRT3) (AA 118-399) protein (His tag)SIRT3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Sirtuin 3 (SIRT3) (AA 297-399) protein (GST tag)SIRT3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sirtuin 3 (SIRT3) (AA 5-257) (Active) protein (His tag)SIRT3 Origin: Mouse Host: Escherichia coli (E. coli) > 90 % CC, AcA Active
Sirtuin 3 (SIRT3) (AA 127-303) (Active) protein (His tag)SIRT3 Origin: Rat Host: Escherichia coli (E. coli) > 95 % CC, AcA Active
Sirtuin 3 (SIRT3) proteinSIRT3 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- SIRT3 (Sirtuin 3 (SIRT3))
- Alternative Name
- sirtuin 3 (SIRT3 Products)
- Synonyms
- zgc:158774 Protein, sirtuin Protein, SIR2L3 Protein, 2310003L23Rik Protein, AI848213 Protein, Sir2l3 Protein, sirtuin 3 Protein, sirt3 Protein, SIRT3 Protein, Sirt3 Protein
- Background
- This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
- Molecular Weight
- 43.4 kDa
- NCBI Accession
- NP_036371
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