PARP1 Protein (Glu988Lys-Mutant) (HA tag,His tag)

Catalog No. ABIN5564467

Product Details

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Glu988Lys-Mutant

Origin: Human

Source: Insect cells (Sf21)

Purification tag / Conjugate: This PARP1 protein is labelled with HA tag,His tag.

Application: SDS-PAGE (SDS)

Cross-Reactivity: Human

Characteristics: Human full-length inactive mutant E998K of PARP-1 is fused to a HA-tag and a His-tag.

Purity: >95 % (SDS-PAGE)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

0.5% of wild type PARP-1.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride and 50 mM imidazole, 0.2 % NP-40 and 10 % glycerol.

Storage: -20 °C,-80 °C

Storage Comment: Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C.

Expiry Date: 6 months

Target Details

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP-1 [ARTD1] (PARP1 Products)

Synonyms: ADPRT Protein, ADPRT 1 Protein, ADPRT1 Protein, ARTD1 Protein, PARP Protein, PARP-1 Protein, PPOL Protein, pADPRT-1 Protein, Adprt Protein, Parp-1 Protein, 5830444G22Rik Protein, AI893648 Protein, Adprp Protein, Adprt1 Protein, C80510 Protein, parp-1 Protein, sPARP-1 Protein, BEST:LD21673 Protein, CG17685 Protein, CG17696 Protein, CG17718 Protein, CG40411 Protein, D.PARP Protein, Dm.pARTa Protein, Dmel\\CG40411 Protein, LD21673.3prime Protein, PARP1 Protein, dPARP Protein, parp Protein, adprt1 Protein, padprt-1 Protein, ppol Protein, si:dkey-206f10.3 Protein, wu:fc60f12 Protein, zgc:110092 Protein, poly(ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase family, member 1 Protein, Poly-(ADP-ribose) polymerase Protein, poly(ADP-ribose) polymerase 1 L homeolog Protein, PARP1 Protein, Parp1 Protein, Parp Protein, parp1.L Protein, parp1 Protein

Background: PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound.

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location