SLIT2 Protein (DYKDDDDK Tag)
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- Target See all SLIT2 Proteins
- SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SLIT2 protein is labelled with DYKDDDDK Tag.
- Application
- SDS-PAGE (SDS)
- Cross-Reactivity
- Human
- Characteristics
- Human Slit2 (aa 1122-1529) is fused at the C-terminus to a FLAG®-tag.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product SLIT2 Protein
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Slit Homolog 2 (Drosophila) (SLIT2) (AA 1361-1460) protein (GST tag)
SLIT2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Slit Homolog 2 (Drosophila) (SLIT2) (AA 1122-1529) protein (His tag)Crystallography grade SLIT2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Slit Homolog 2 (Drosophila) (SLIT2) (AA 1114-1521) protein (His tag)Crystallography grade SLIT2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Slit Homolog 2 (Drosophila) (SLIT2) (AA 31-1121) protein (His tag)Crystallography grade SLIT2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Slit Homolog 2 (Drosophila) (SLIT2) (AA 31-1529) protein (His tag)Crystallography grade SLIT2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Slit Homolog 2 (Drosophila) (SLIT2) (AA 26-1113) protein (His tag)Crystallography grade SLIT2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Slit Homolog 2 (Drosophila) (SLIT2) (AA 26-1521) protein (His tag)Crystallography grade SLIT2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Activates an UCP1-luciferase construct in mouse fat primary cells at a concentration of 20nM.
- Restrictions
- For Research Use only
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- Format
- Solid
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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- Target
- SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))
- Alternative Name
- Slit2 (SLIT2 Products)
- Synonyms
- SLIL3 Protein, Slit-2 Protein, Drad-1 Protein, E030015M03Rik Protein, E130320P19Rik Protein, Slil3 Protein, mKIAA4141 Protein, Slit Protein, slil3 Protein, slit-2 Protein, slit2-a Protein, SLIT2 Protein, slit guidance ligand 2 Protein, slit homolog 2 (Drosophila) Protein, slit guidance ligand 2 S homeolog Protein, SLIT2 Protein, Slit2 Protein, slit2 Protein, slit2.S Protein
- Background
- The Slits are secreted proteins that bind to Robo receptors and play a role in axon guidance and neuronal migration. The Slit family in mice and humans comprises three members: Slit1, Slit2 and Slit3. These are all extracellular matrix proteins of 180-200 kDa. In vivo, Slit2 is cleaved into 140 kDa N-terminal (Slit2-N) and 55-60 kDa C-terminal (Slit2-C) fragments. Only Slit2-N and Slit2 full-length bind the Robo proteins. Recently, it has been shown that Slit2 is highly expressed in adipose tissues under the control of PRDM16 and cold exposure. Slit2 is posttranslationally cleaved by adipocytes and the resulting Slit2-C fragment improves glucose homeostasis in mice by activating a thermogenic PKA/cAMP pathway not mediated by beta-adrenergic receptors in adipocytes.
- Molecular Weight
- ~55kDa
- UniProt
- O94813
- Pathways
- Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration
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