ZEBOV GP Protein (partial) (His tag)

Catalog No. ABIN5608205

Recombinant ZEBOV GP protein expressed in HEK-293 Cells.

Quick Overview for ZEBOV GP Protein (partial) (His tag) (ABIN5608205)

Target: ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))

Protein Type: Recombinant

Origin: Ebola Virus, Zaire ebolavirus

Source: HEK-293 Cells

Application: Western Blotting (WB)

Purity: >85 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: partial

Purification tag / Conjugate: This ZEBOV GP protein is labelled with His tag.

Sequence: Ile 33 - Asp 637

Endotoxin Level: Endotoxin level is less than 1.0 EU per ug by the LAL method.

Application Details

Application Notes: This recombinant protein can be used for WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.

Target Details

Target: ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))

Alternative Name: ZEBOV GP

Target Type: Viral Protein

Background: EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160- kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

Molecular Weight: 67 kDa

Gene ID: 279888

UniProt: P87666