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- Target See all COL3 Proteins
- COL3 (Collagen, Type III (COL3))
- Protein Type
- Native
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Origin
- Human
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Source
- Human
- Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Purification
- Human Collagen type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type III. Reaction with Rockland's anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
- Sterility
- Sterile filtered
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- Application Notes
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Immunohistochemistry Dilution: 1:100-1:500
Application Note: Human Collagen type III purified protein standard is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.5 M Acetic Acid
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 6 months
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- Target
- COL3 (Collagen, Type III (COL3))
- Alternative Name
- Collagen Type III (COL3 Products)
- Synonyms
- PT Protein, RPRGL2 Protein, THPH1 Protein, Cf-2 Protein, Cf2 Protein, FII Protein, thrombin Protein, prothrombin Protein, coagulation factor II, thrombin Protein, coagulation factor II Protein, F2 Protein
- Gene ID
- 1281
- UniProt
- P02461
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