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ARSF Protein (AA 23-300) (His tag)

ARSF Origin: Human Host: Escherichia coli (E. coli) > 97 % CC, AcA Active
Catalog No. ABIN5665766
  • Target See all ARSF Proteins
    ARSF (Arylsulfatase F (ARSF))
    Biological Activity
    Active
    Protein Characteristics
    AA 23-300
    Origin
    Human
    Source
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This ARSF protein is labelled with His tag.
    Application
    Cell Culture (CC), Activity Assay (AcA)
    Characteristics
    Tag location: N-terminal His Tag
    Purity
    > 97 %
    Biological Activity Comment
    ARSF (Arylsulfatase F) is a member of the sulfatase gene family, and more specifically, the arylsulfatase subfamily. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Besides, EP300 (E1A binding protein p300) has been identified as an interactor of ARSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSF and recombinant human EP300. Briefly, ARSF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ARSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSF and EP300 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ARSF with EP300.
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    Discover our top product ARSF Protein
  • Application Notes
    Isoelectric Point: 6.9
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Preservative
    Dithiothreitol (DTT), Other preservative, ProClin
    Precaution of Use
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    ARSF (Arylsulfatase F (ARSF))
    Abstract
    ARSF Products
    Synonyms
    ASF Protein, ARSF Protein, arylsulfatase F Protein, ARSF Protein
    Background
    Alternative Names: ASF
    Molecular Weight
    35kDa
    UniProt
    P54793
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