Cathepsin L2 Protein (CTSL2) (AA 64-334) (His tag)
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- Target See all Cathepsin L2 (CTSL2) Proteins
- Cathepsin L2 (CTSL2)
- Biological Activity
- Active
- Protein Characteristics
- AA 64-334
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Cathepsin L2 protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 95 %
- Biological Activity Comment
- Cathepsin V (CTSV) is a lysosomal cysteine proteinase which belongs to peptidase C1 family. It is expressed in normal human thymus, testis and corneal epithelium. Cathepsin V plays an important role in corneal physiology and mediates degradation of invariant chain in human thymus. Besides, Retinoblastoma Protein 1 (RB1) has been identified as an interactor of CTSV, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CTSV and recombinant human RB1. Briefly, CTSV were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RB1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CTSV pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CTSV and RB1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CTSV with RB1.
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Cathepsin L2 (CTSL2) (AA 64-334) (Active) protein (His tag)CTSL2 Origin: Human Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
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- Application Notes
- Isoelectric Point: 8.8
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- Cathepsin L2 (CTSL2)
- Alternative Name
- Cathepsin V (CTSV) (CTSL2 Products)
- Synonyms
- CATL2 Protein, CTSL2 Protein, CTSU Protein, CTSL Protein, CTSL1 Protein, ctsl2 Protein, LOC100232421 Protein, cathepsin V Protein, cathepsin V L homeolog Protein, cathepsin L Protein, CTSV Protein, ctsv.L Protein, ctsv Protein, CTSL Protein, Ctsv Protein
- Background
- Alternative Names: CTS-L2, CTSL2, CATL2, CTSU, CTS-V, Cathepsin L2
- Molecular Weight
- 32kDa
- UniProt
- O60911
- Pathways
- Toll-Like Receptors Cascades
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