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CLCA1 Protein (AA 416-670) (His tag)

CLCA1 Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Catalog No. ABIN5665774
  • Target See all CLCA1 Proteins
    CLCA1 (Chloride Channel Accessory 1 (CLCA1))
    Biological Activity
    Active
    Protein Characteristics
    AA 416-670
    Origin
    • 4
    • 1
    Human
    Source
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This CLCA1 protein is labelled with His tag.
    Application
    Activity Assay (AcA), Cell Culture (CC)
    Characteristics
    Tag location: N-terminal His Tag
    Purity
    > 97 %
    Biological Activity Comment
    CLCA1 (Calcium-activated chloride channel regulator 1) is a member of the calcium sensitive chloride conductance protein family. This protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits. It has been reported that CLCA1 activates calcium-dependent chloride channel through the interaction with TMEM16A (anoctamin-1). Besides, there exits similarities between human and mouse TMEM16A in amino acid sequence with the identity of 89.66%. Thus, a functional ELISA assay was conducted to detect the association of recombinant human CLCA1 with recombinant mouse TMEM16A. Briefly, CLCA1 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TMEM16A-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CLCA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CLCA1 with TMEM16A was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of CLCA1 with TMEM16A
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  • Application Notes
    Isoelectric Point: 7.1
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
    Preservative
    Dithiothreitol (DTT), ProClin
    Precaution of Use
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    CLCA1 (Chloride Channel Accessory 1 (CLCA1))
    Abstract
    CLCA1 Products
    Synonyms
    CACC Protein, CACC1 Protein, CLCRG1 Protein, CaCC-1 Protein, GOB5 Protein, hCLCA1 Protein, hCaCC-1 Protein, Clca3 Protein, fi39d11 Protein, wu:fi39d11 Protein, AECC Protein, Clca1 Protein, Clca2 Protein, gob-5 Protein, gob5 Protein, chloride channel accessory 1 Protein, chloride channel Protein, chloride channel protein Protein, CLCA1 Protein, Clca1 Protein, clca1 Protein, clcA1 Protein
    Background
    Alternative Names: CaCC1, CLCRG1, Chloride Channel,Calcium Activated,Family Member 1, Calcium-activated chloride channel protein 1
    Molecular Weight
    28kDa
    UniProt
    A8K7I4
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