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Hexosaminidase A Protein (HEXA) (AA 319-528) (His tag)

HEXA Origin: Mouse Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Catalog No. ABIN5665811
  • Target See all Hexosaminidase A (HEXA) Proteins
    Hexosaminidase A (HEXA)
    Biological Activity
    Active
    Protein Characteristics
    AA 319-528
    Origin
    • 5
    • 3
    • 1
    • 1
    Mouse
    Source
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This Hexosaminidase A protein is labelled with His tag.
    Application
    Activity Assay (AcA), Cell Culture (CC)
    Characteristics
    Tag location: N-terminal His Tag
    Purity
    > 97 %
    Biological Activity Comment
    Hexosaminidase A Alpha (HEXa) is a lysosomal enzyme. There are three predominant isoenzymes: hexosaminidase A, B and S. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines. The enzymes are composed of two alpha and/or beta subunits, which are coded by HEXA and HEXB genes, respectively. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit which contains a key residue, Arg-424 is able to hydrolyze GM2 gangliosides. Hexosaminidase A (alpha polypeptide) plays a critical role in the brain and spinal cord (central nervous system). Besides, Hexosaminidase B Beta (HEXB) has been identified as an interactor of HEXA, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse HEXA and recombinant mouse HEXB. Briefly, HEXA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HEXB-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HEXA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of HEXA and HEXB was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of HEXA with HEXB.
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  • Application Notes
    Isoelectric Point: 6.3
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Preservative
    Dithiothreitol (DTT), Other preservative, ProClin
    Precaution of Use
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    Hexosaminidase A (HEXA)
    Alternative Name
    Hexosaminidase A Alpha (HEXa) (HEXA Products)
    Synonyms
    TSD Protein, fc04h11 Protein, wu:fc04h11 Protein, wu:fv09f06 Protein, si:dkey-35i22.2 Protein, HEXA Protein, Hex-1 Protein, zgc:112084 Protein, hexosaminidase subunit alpha Protein, hexosaminidase B (beta polypeptide) Protein, CUGBP Elav-like family member 6 Protein, hexosaminidase A Protein, hexosaminidase A (alpha polypeptide) Protein, beta-hexosaminidase subunit alpha-like Protein, HEXA Protein, hexb Protein, CELF6 Protein, Hexa Protein, hexa Protein, LOC100228061 Protein
    Background
    Alternative Names: TSD, Hex-A, Tay Sachs Disease, GM2 Gangliosidosis, Beta-Hexosaminidase Subunit Alpha, Beta-N-Acetylhexosaminidase Subunit Alpha, N-Acetyl-Beta-Glucosaminidase Alpha
    Molecular Weight
    28kDa
    UniProt
    P29416
    Pathways
    Sensory Perception of Sound, Glycosaminoglycan Metabolic Process
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