HRG Protein (AA 19-525) (His tag)
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- Target See all HRG Proteins
- HRG (Histidine-Rich Glycoprotein (HRG))
- Biological Activity
- Active
- Protein Characteristics
- AA 19-525
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HRG protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- HRG (Histidine-rich glycoprotein) is a plasma glycoprotein which is distinguished by its high content of histidine and proline. HRG binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Thus, a functional binding ELISA assay was constructed to detect the association of rhHRG with heparin. Briefly, Microtiter wells were coated with OVA-conjugated-heparin. rhHRG were diluted serially in 0.01M PBS (pH 7.4). Duplicate samples of 100uL were then transferred to the coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HRG PAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate Solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of rhHRG with heparin was shown in Figure 1 and this effect was in a dose dependent manner The Binding Activity of rhHRG with Heparin.
- Top Product
- Discover our top product HRG Protein
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Histidine-Rich Glycoprotein (HRG) (AA 1-525) protein (GST tag)
HRG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Histidine-Rich Glycoprotein (HRG) (AA 19-525) protein (His tag)Crystallography grade HRG Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Histidine-Rich Glycoprotein (HRG) (AA 19-525) protein (His tag)Crystallography grade HRG Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Isoelectric Point: 7
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- HRG (Histidine-Rich Glycoprotein (HRG))
- Alternative Name
- Histidine Rich Glycoprotein (HRG) (HRG Products)
- Synonyms
- AI265597 Protein, AW413091 Protein, D16jh2 Protein, D18020 Protein, Hprg Protein, Hrgp Protein, HPRG Protein, HRGP Protein, THPH11 Protein, HRG1 Protein, histidine rich glycoprotein Protein, histidine-rich glycoprotein Protein, HRG Protein, LOAG_12427 Protein, LOC100597044 Protein, Hrg Protein
- Background
- Alternative Names: HPRG, HRGP, Histidine-Proline Rich Glycoprotein, Thrombophilia Due To Elevated HRG
- Molecular Weight
- 75kDa
- UniProt
- P04196
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