IFNA Protein (AA 24-189) (His tag)
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- Target See all IFNA Proteins
- IFNA (Interferon alpha (IFNA))
- Biological Activity
- Active
- Protein Characteristics
- AA 24-189
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IFNA protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- The Interferon-alpha (IFNa) proteins belong to type I interferons (IFNs) which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins produced by leukocytes are also known as leukocyte interferon. They are mainly involved in innate immune response against viral infection. Besides, Interferon-alpha 13 (IFNA13) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IFNa and recombinant mouse IFNA13. Briefly, IFNA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNA13-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IFNapAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNA13 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of IFNa with IFNA13.
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Interferon alpha (IFNA) protein (His-Avi Tag)
IFNA Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interferon alpha (IFNA) protein (His-Avi Tag,Biotin)IFNA Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interferon alpha (IFNA) (AA 27-243) protein (His tag)IFNA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon alpha (IFNA) (AA 27-429) protein (His-Avi Tag)IFNA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon alpha (IFNA) (AA 27-429) protein (Fc Tag)IFNA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Application Notes
- Isoelectric Point: 9.1
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- IFNA (Interferon alpha (IFNA))
- Abstract
- IFNA Products
- Synonyms
- IFN-alphaO Protein, IFN-ALPHA-1 Protein, IFN1@ Protein, Ifa Protein, Ifa8 Protein, interferon alpha 16 Protein, interferon, alpha 1 Protein, interferon alpha Protein, interferon-alpha Protein, IFNA16 Protein, IFNA1 Protein, Ifna Protein, IFNA Protein
- Background
- Alternative Names: IFNA1, IFL, LeIF D, IFN, IFN-Alpha, IFNA13, IFN-A, IFNAP22, IFN-Alpha 1b, Interferon Alpha 1b, Interferon, Leukocytic, IFN, Leukocyte, Interferon alpha-D
- Molecular Weight
- 20/25kDa
- UniProt
- P01572
- Pathways
- JAK-STAT Signaling, TLR Signaling, Hepatitis C, Inflammasome
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