IL1F9 Protein (AA 1-162) (His tag)

Catalog No. ABIN5665829

Product Details

Target: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Biological Activity: Active

Protein Characteristics: AA 1-162

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This IL1F9 protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 90 %

Biological Activity Comment: IL36G (Interleukin 36 gamma), also known as IL-1F9 and IL-1H1 is a member of the IL-1 family. The receptor for IL36 gamma is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human IL36G with recombinant human IL1R1. Briefly, IL36G were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL36G with IL1R1 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL36G with IL1R1.

Application Details

Application Notes: Isoelectric Point: 6.3

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Alternative Name: Interleukin 1 Family, Member 9 (IL1F9) (IL1F9 Products)

Synonyms: IL-1F9 Protein, IL-1H1 Protein, IL-1RP2 Protein, IL1E Protein, IL1F9 Protein, IL1H1 Protein, IL1RP2 Protein, Il36g Protein, Il1f9 Protein, RGD1563019 Protein, IL36G Protein, interleukin 36 gamma Protein, interleukin 1 family, member 9 Protein, interleukin 36, gamma Protein, interleukin-36 gamma Protein, IL36G Protein, Il1f9 Protein, Il36g Protein, LOC100065031 Protein

Background: Alternative Names: IL-1F9, IL36g, IL-1H1, IL-1RP2, IL1E, IL1H1, IL1RP2, Interleukin 36, Gamma, Interleukin-1 Homolog 1, Interleukin-1 epsilon, Interleukin 1-Related Protein 2

Molecular Weight: 19kDa

UniProt: Q9NZH8