PON1 Protein (AA 35-206) (His tag)
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- Target See all PON1 Proteins
- PON1 (Paraoxonase 1 (PON1))
- Biological Activity
- Active
- Protein Characteristics
- AA 35-206
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PON1 protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- Paraoxonase 1 (PON1) is responsible for hydrolysing organophosphate pesticides and nerve gasses. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). Besides, Clusterin (CLU) has been identified as an interactor of PON1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON1 and recombinant human CLU. Briefly, PON1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CLU-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PON1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of PON1 and CLU was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of PON1 with CLU.
- Top Product
- Discover our top product PON1 Protein
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- Application Notes
- Isoelectric Point: 5.1
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- PON1 (Paraoxonase 1 (PON1))
- Abstract
- PON1 Products
- Synonyms
- esa Protein, pon Protein, pon2 Protein, MGC53915 Protein, MGC89222 Protein, PON1 Protein, ESA Protein, MVCD5 Protein, PON Protein, Pon Protein, paraoxonase 2 Protein, paraoxonase 1 Protein, pon2 Protein, PON1 Protein, Pon1 Protein
- Background
- Alternative Names: ESA, PON, Esterase A, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, A-esterase 1, Serum aryldialkylphosphatase 1
- Molecular Weight
- 30kDa
- UniProt
- P27169
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