PTH Protein (AA 32-115) (MBP tag,His tag)

Catalog No. ABIN5665891

Product Details

Target: PTH (Parathyroid Hormone (PTH))

Biological Activity: Active

Protein Characteristics: AA 32-115

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PTH protein is labelled with MBP tag,His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: Two N-terminal Tags, His-tag and MBP-tag

Purity: > 90 %

Biological Activity Comment: PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is important in bone remodeling. As reported, osteoblast-like cell lines, such as ROS 17/2.8, UMR106, SaOS, U2OS, MG63, that exhibit PTHR1, respond with increased proliferation to PTH. Rat PTH shares similarities with human PTH in amino acid sequence with the identity of 71.3%. Thus, a proliferation assay of rat recombinant PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of U2OS cells after incubation with PTH for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant PTH for 48h. The result was shown in Figure 2. It was obvious that PTH increased cell viability of U2OS cells.

Application Details

Application Notes: Isoelectric Point: 5.8

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: PTH (Parathyroid Hormone (PTH))

Abstract: PTH Products

Synonyms: PTH1 Protein, Pthp Protein, PTH-(1-84) Protein, Pth1 Protein, Pthr1 Protein, PTH Protein, parathyroid hormone Protein, parathyroid hormone S homeolog Protein, PTH Protein, Pth Protein, pth.S Protein

Target Type: Hormone

Background: Alternative Names: iPTH, Intact Parathyroid Hormone, Parathormone, Parathyrin

Molecular Weight: 59kDa

UniProt: P04089

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process