LMYC Protein (AA 184-454) (His tag,GST tag)

Catalog No. ABIN5665922

Product Details

Target: LMYC (MYCL) (Protein L-Myc (MYCL))

Biological Activity: Active

Protein Characteristics: AA 184-454

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This LMYC protein is labelled with His tag,GST tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His and GST Tag

Purity: > 97 %

Biological Activity Comment: MYC (Myc proto-oncogene protein) is a nuclear phosphoprotein that binds specific sequence of DNA. MYC functions as a transcription factor and regulates transcription of target genes. It has been proven that c-Myc protein is intracellularly associated with TBP (TATA-binding protein) of the TFIID transcription initiation complex; besides, TRRAP (Transformation/transcription domain-associated protein) is thought to be an essential cofactor for the MYC. Thus a binding ELISA assay was conducted to detect the interaction of MYC with TBP and TRRAP. Briefly, recombinant human MYC were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TBP-coated and TRRAP microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MYC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MYC with TBP and TRRAP was shown in Figure 1 and Figure 2 separately, and this effect was in a dose dependent manner The binding activity of MYC with TBP. Figure 2. The binding activity of MYC with TRRAP

Application Details

Application Notes: Isoelectric Point: 6.6

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: LMYC (MYCL) (Protein L-Myc (MYCL))

Alternative Name: V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) (MYCL Products)

Synonyms: MRTL Protein, MYCC Protein, bHLHe39 Protein, c-Myc Protein, MYCL1 Protein, Lmyc1 Protein, Mycl1 Protein, LMYC Protein, bHLHe38 Protein, CMYC Protein, c-myc Protein, ARC Protein, B430311C09Rik Protein, MYC Protein, NOP Protein, Nop30 Protein, RNCMYC Protein, mMyc Protein, C-MYC Protein, AU016757 Protein, Myc2 Protein, Niard Protein, Nird Protein, AW536278 Protein, L-myc Protein, Lmyc-1 Protein, l-myc Protein, lmyc Protein, lmyc-b Protein, lmyc2 Protein, mycl1-b Protein, xL-Myc2 Protein, fi04c04 Protein, mycl1b Protein, si:ch211-194e15.2 Protein, wu:fa07c04 Protein, wu:fb19f03 Protein, wu:fi04c04 Protein, zgc:152988 Protein, lmyc1 Protein, mycl1 Protein, mycl1a Protein, zgc:85967 Protein, lmyc-A Protein, mycl Protein, mycl1-a Protein, xL-Myc1 Protein, xL-myc1 Protein, MYC proto-oncogene, bHLH transcription factor Protein, v-myc avian myelocytomatosis viral oncogene lung carcinoma derived homolog Protein, MYCL proto-oncogene, bHLH transcription factor Protein, nucleolar protein 3 (apoptosis repressor with CARD domain) Protein, myelocytomatosis oncogene Protein, v-myc avian myelocytomatosis viral oncogene lung carcinoma derived Protein, MYCL proto-oncogene, bHLH transcription factor S homeolog Protein, MYCL proto-oncogene, bHLH transcription factor b Protein, MYCL proto-oncogene, bHLH transcription factor a Protein, MYCL proto-oncogene, bHLH transcription factor L homeolog Protein, MYC Protein, MYCL Protein, Mycl Protein, Nol3 Protein, Myc Protein, mycl.S Protein, myclb Protein, mycla Protein, mycl.L Protein

Background: Alternative Names: C-Myc, BHLHE39, Myc Proto-Oncogene Protein, Class E basic helix-loop-helix protein 39, Transcription factor p64, Proto-oncogene c-Myc

Molecular Weight: 65kDa

UniProt: P01106

Pathways: Warburg Effect