AIF Protein (AA 103-612, partial) (His-SUMO Tag)

Catalog No. ABIN5709025

Product Details

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Protein Type: Recombinant

Protein Characteristics: AA 103-612, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This AIF protein is labelled with His-SUMO Tag.

Application: SDS-PAGE (SDS)

Sequence: GLTPEQKQKK AALSASEGEE VPQDKAPSHV PFLLIGGGTA AFAAARSIRA RDPGARVLIV SEDPELPYMR PPLSKELWFS DDPNVTKTLR FKQWNGKERS IYFQPPSFYV SAQDLPHIEN GGVAVLTGKK VVQLDVRDNM VKLNDGSQIT YEKCLIATGG TPRSLSAIDR AGAEVKSRTT LFRKIGDFRS LEKISREVKS ITIIGGGFLG SELACALGRK ARALGTEVIQ LFPEKGNMGK ILPEYLSNWT MEKVRREGVK VMPNAIVQSV GVSSGKLLIK LKDGRKVETD HIVAAVGLEP NVELAKTGGL EIDSDFGGFR VNAELQARSN IWVAGDAACF YDIKLGRRRV EHHDHAVVSG RLAGENMTGA AKPYWHQSMF WSDLGPDVGY EAIGLVDSSL PTVGVFAKAT AQDNPKSATE QSGTGIRSES ETESEASEIT IPPSTPAVPQ APVQGEDYGK GVIFYLRDKV VVGIVLWNIF NRMPIARKII KDGEQHEDLN EVAKLFNIHE

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1 (AIFM1 Products)

Synonyms: AIF Protein, CMTX4 Protein, COWCK Protein, COXPD6 Protein, PDCD8 Protein, CG7263 Protein, DmAIF Protein, Dmel\\CG7263 Protein, GB16024 Protein, DDBDRAFT_0187853 Protein, DDBDRAFT_0191137 Protein, DDB_0187853 Protein, DDB_0191137 Protein, aif Protein, pdcd8 Protein, AIFM1 Protein, PCD8 Protein, AIFsh2 Protein, Hq Protein, Pdcd8 Protein, mAIF Protein, Aif Protein, zgc:91994 Protein, apoptosis inducing factor mitochondria associated 1 Protein, allograft inflammatory factor 1 Protein, Apoptosis inducing factor Protein, apoptosis-inducing factor 1, mitochondrial Protein, apoptosis inducing factor Protein, apoptosis inducing factor, mitochondria associated 1 Protein, apoptosis-inducing factor, mitochondrion-associated, 1 Protein, apoptosis-inducing factor, mitochondrion-associated 1 Protein, AIFM1 Protein, AIF1 Protein, AIF Protein, LOC412212 Protein, aif Protein, aifm1 Protein, Aifm1 Protein

Background: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermbrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Molecular Weight: 71.5 kDa

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect